High Speed Imaging at and Beyond the Diffraction Limit
Speaker: Hari Shroff, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health
Keywords: Structured illumination microscopy, light sheet microscopy, adaptive optics, neurodevelopment, developmental biology
I will discuss our efforts to improve structured illumination microscopy (SIM) and light-sheet microscopy. SIM doubles the spatial resolution of light microscopy, requiring lower light intensities and acquisition times than other super-resolution techniques. I will present SIM implementations that enable resolution doubling in live samples > 10-20x thicker than possible with conventional SIM, as well as hardware modifications that enable effectively ‘instant’ SIM imaging at rates 10-100x faster than other SIM. New applications of instant SIM, including combination with total internal reflection (TIRF) and with adaptive optics will also be discussed. The second half of the talk will focus on the development of inverted selective plane illumination microscopy (iSPIM), and subsequent application to the noninvasive study of neurodevelopment in nematodes. I will discuss progress that quadruples the axial resolution of iSPIM by using a second specimen view, enabling imaging with isotropic spatial resolution (dual-view iSPIM, or diSPIM). Newer multiview microscopes with more objectives and more views, further improving spatial resolution, will also be described. Applications of these technologies will be presented, including computational methods for untwisting worm embryos and calcium imaging in freely moving embryos.
Host: Tristan Ursell
Location: 100 Willamette Hall
Reception from 3:40-3:55 Willamette Hall Paul Olum Atrium